多糖-硒化多糖复方抗氧化活性的比较

doi:10.11843/j.issn.0366-6964.2017.02.020

摘要/Abstract

摘要：

为了研制抗氧化中药多糖制剂，在前期研究的基础上，选择抗氧化活性较强的枸杞多糖（LBP）、白术多糖（AMP）、当归多糖（CAP）、党参多糖（CPP）、大蒜多糖（GP）、百合多糖（LP）、五味子多糖（SCP）、硒化枸杞多糖（sLBP）、硒化白术多糖（sAMP）、硒化当归多糖（sCAP）为组分药，以糖含量按照多糖-硒化多糖9∶1的比例组成18个复方，比较它们的抗氧化活性。体外试验，比较18个复方对3种自由基的清除能力；体内试验，雏鸡免疫新城疫苗时分别注射高、低剂量的3个复方，测定血清3种抗氧化酶和MDA含量的动态变化。体外试验结果显示，18个复方的5个浓度对DPPH自由基、羟自由基和ABTS自由基均有不同程度的清除能力，LBP-sAMP、CAP-sLBP和CAP-sAMP的能力较强。体内试验结果显示，6个复方组在4个时间点的血清CAT、SOD、GSH-Px活性多高于或显著高于对照组，MDA含量均低于（多显著低于）对照组；CAP-sLBP组的3种抗氧化酶活性均为最高、MDA含量最低。18个复方均有不同程度的抗氧化活性，CAP-sLBP在18个复方中的抗氧化活性最强，可以考虑作为多糖类抗氧化制剂的候选方。

Abstract:

In order to develop the antioxidant polysaccharide preparations, based on our previous researches, 18 compounds composed with Lycium barbarum polysaccharide (LBP), Atractylodes macrocephala polysaccharide (AMP), Chinese angelica polysaccharide (CAP), Codonopsis pilosula polysaccharide (CPP), Garlic polysaccharide (GP), Lily polysaccharide (LP), Schisandra chinensis polysaccharide (SCP), selenizing LBP (sLBP), selenizing AMP (sAMP), and selenizing CAP (sCAP) with stronger antioxidant activities according to carbohydrate content at 9:1 ratio of polysaccharide to selenizing polysaccharide were chose. Their antioxidant activities were compared. In vitro tests, the scavenging abilities of 18 compounds were compared for 3 kinds of free radicals. In vivo tests, the chicks were injected respectively with 3 compounds each at high and low dosage when they were vaccinated with Newcastle disease vaccine, and the dynamic changes of serum contents of 3 antioxidases and MDA were determined. The results of in vitro tests showed that 18 compounds each at 5 concentrations had different extent scavenging abilities for DPPH radical, hydroxyl radical and ABTS radicals, and the abilities of LBP-sAMP, CAP-sLBP, and CAP-sAMP were stronger. The results of in vivo test showed that in 6 compound groups at 4 time points, the activities of CAT, SOD and GSH-Px were higher or significantly higher than those in control group, and MDA contents were lower or significantly lower than those in control group. In CAP-sLBP group, 3 antioxidases activities were the highest and MDA contents were the lowest. These results indicate that 18 compounds possess differen extent antioxidant activities, the activity of CAP-sLBP is the strongest among 18 compounds and can be considered as the candidate of the antioxidant polysaccharide preparation.

中图分类号:

S853.74

田卫军，刘宽辉，高珍珍，侯冉冉，岳婵娟，秦嘉玲，王德云，胡元亮. 多糖-硒化多糖复方抗氧化活性的比较[J]. 畜牧兽医学报, 2017, 48(2): 359-368.

TIAN Wei-jun, LIU Kuan-hui, GAO Zhen-zhen, HOU Ran-ran, YUE Chan-juan, QIN Jia-ling, WANG De-yun, HU Yuan-liang. The Antioxidant Activity Comparison of Polysaccharide-selenizing Polysaccharide Compounds[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(2): 359-368.

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https://www.xmsyxb.com/CN/Y2017/V48/I2/359

参考文献

［1］刘成梅, 游海. 天然产物有效成分的分离与应用［M］. 北京: 化学工业出版社, 2003.
LIU C M, YOU H. Separation and application of effective components from natural products ［M］. Beijing: Chemical Industry Press, 2003.（in Chinese）
［2］BIRRINGER M, PILAWA S, FLOHÉ L. Trends in selenium biochemistry ［J］. Nat Prod Rep, 2002, 19(6): 693-718.
［3］徐辉碧, 黄开勋，高中洪. 硒蛋白与硒多糖［J］.科学, 1997, 49(5): 22-25.
XU H B, HUANG K X, GAO Z H. Selenoproteins and selenopolysaccharides ［J］. Science, 1997, 49(5): 22-25. (in Chinese)
［4］黄峙, 郑文杰, 郭宝江. 含硒生物大分子化合物研究进展［J］. 海南大学学报(自然科学版), 2001, 19(2): 169-175.
HUANG Z, ZHENG W J, GUO B J. Research progress on Se-containing biomacromolecules ［J］. Natural Science Journal of Hainan University (Natural Science Edition), 2001, 19(2): 169-175. (in Chinese)
［5］STAAF M, YANG Z, HUTTUNEN E, et al. Structural elucidation of the viscous exopolysaccharide produced by Lactobacillus helveticus Lb161 ［J］. Carbohydr Res, 2000, 326(2): 113-119.
［6］高玉杰，吕海涛. 浒苔硒多糖的制备及其活性研究［J］. 化学与生物工程, 2012, 29(5): 40-45.
GAO Y J, LÜ H T.Study on preparation and activities of selenium Enteromorpha polysaccharides ［J］. Chemistry & Bioengineering, 2012, 29(5): 40-45. (in Chinese)
［7］YUE C, CHEN J, HOU R, et al. Effects of selenylation modification on antioxidative activities of Schisandra chinensis polysaccharide［J］.PLoS One,2015,10(7):e0134363.
［8］QIN T, CHEN J, WANG D, et al. Selenylation modification can enhance immune-enhancing activity of Chinese angelica polysaccharide ［J］. Carbohydr Polym, 2013, 95(1): 183-187.
［9］QIU S, CHEN J, CHEN X, et al. Optimization of selenylation for lycium barbarum polysaccharide based on antioxidant activity ［J］. Carbohydr Polym, 2014, 103: 148-153.
［10］LIU J, CHEN X, YUE C J, et al. Effect of selenylation modification on immune-enhancing activity of Atractylodes macrocephala polysaccharide ［J］. Int J Biol Macromol, 2015, 72: 1435-1440.
［11］QIN T, CHEN J, WANG D, et al. Optimization of selenylation conditions for Chinese angelica polysaccharide based on immune-enhancing activity ［J］. Carbohydrate Polymers, 2013, 92(1): 645-650.
［12］孙仕玲. 山药多糖含量的测定方法研究［J］. 农业与技术, 2010, 30(3): 35-39.
SUN S L. Study on content determination method of Yam polysaccharide ［J］. Agriculture & Technology, 2010, 30(3): 35-39.(in Chinese)
［13］高建忠, 秦顺义, 黄克和. 氢化物发生-原子荧光光谱法测定富硒酵母中的有机硒和无机硒［J］. 分析科学学报, 2006, 22(2): 157-160.
GAO J Z, QIN S Y, HUANG K H. Determination of the organic and inorganic selenium contents in selenium-enriched yeast by hydride generation-atomic fluorescence spectrometry ［J］. Journal of Analytical Science, 2006, 22(2): 157-160.(in Chinese)
［14］MARX J L.Oxygen free radicals linked to many diseases［J］. Science, 1987, 235(4788): 529-531.
［15］李厚勇, 王蕊, 高晓奇, 等. 海带提取物对脂质过氧化和血液流变学的影响［J］. 中国公共卫生, 2002, 18(3): 263-264.
LI H Y, WANG R, GAO X Q, et al. Effect of laminaria japonica-extract on peroxidation and hemorheology ［J］. China Public Health, 2002, 18(3): 263-264.(in Chinese)
［16］陈茜, 邢玉娟，陈玉库，等. 黄芪多糖和氧化苦参碱协同增强鸡新城疫疫苗免疫效果研究［J］. 西北农林科技大学学报（自然科学版）, 2015, 43(6): 41-46.
CHEN Q, XING Y J, CHEN Y K, et al. Increasing immune efficacy of newcastle disease vaccine in chicken by astragalus polysaccharide and oxymatrine ［J］. Journal of Northwest A & F University (Natural Science Edition), 2015, 43(6): 41-46.(in Chinese)
［17］YOKOZAWA T, DONG E, NAKAGAWA T, et al. In vitro and in vivo studies on the radical-scavenging activity of tea［J］. J Agric Food Chem, 1998, 46(6): 2143-2150.
［18］张丽萍, 童华荣. 抗氧化能力测定方法的研究进展［J］. 中国食品添加剂, 2004(3): 108-113.
ZHANG L P, TONG H R. Advanced studies on the methods for antioxidants screening ［J］. China Food Additives, 2004(3): 108-113.(in Chinese)
［19］MILLER N J, RICE-EVANS C, DAVIES M J, et al. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates［J］. Clin Sci(Lond), 1993, 84(4): 407-412.
［20］CHANCE B, SIES H, BOVERIS A. Hydroperoxide metabolism in mammalian organs ［J］. Physiol Rev, 1979, 59(3): 527-605.
［21］王翠菊, 王洪芳, 陈辉，等. 黄芪多糖对蛋鸡抗氧化性能和蛋品质的影响［J］. 动物营养学报, 2011, 23(2): 280-284.
WANG C J, WANG H F, CHEN H, et al. Effects of Astragalus polysaccharides on antioxidant capacity and egg quality of laying hens ［J］. Chinese Journal of Animal Nutrition, 2011, 23(2): 280-284. (in Chinese)
［22］SUNDE R A. mRNA transcripts as molecular biomarkers in medicine and nutrition ［J］. J Nutr Biochem, 2010, 21(8): 665-670.
［23］王健，龚兴国.多糖的抗肿瘤及免疫调节研究进展［J］.中国生化药物杂志，2001，22（1）：52-54.
WANG J， GONG X G. Advance in the studies on the antitumor activity and immunomodulating action of polysaccharides ［J］. Chinese Journal of Biochemical Pharmaceuticals, 2001, 22(1): 52-54.（in Chinese）
［24］温悦, 傅正毅, 赖艳, 等. 当归多糖的药理作用研究进展［J］. 中国医药导报, 2012, 9(30): 27-29.
WEN Y, FU Z Y, LAI Y, et al. Research progress of pharmacological action of angelica polysaccharides ［J］. China Medical Herald, 2012, 9(30): 27-29. (in Chinese)
［25］李越鲲, 周旋, 刘兰英, 等. 不同分子量枸杞多糖体外抗氧化活性研究［J］. 安徽农业科学, 2012, 40(29): 14174-14175, 14239.
LI Y K, ZHOU X, LIU L Y, et al. Antioxidation in vitro of polysaccharides with different molecular weight from Lycium bararum L. ［J］． Journal of Anhui Agricultural Sciences, 2012, 40(29): 14174-14175, 14239. (in Chinese)

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